| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.008231 |
| Chromosome: | chromosome 6 |
| Location: | 321370 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g800592 | (1 of 1) PF05551 - Zinc-binding loop region of homing endonuclease (zf-His_Me_endon) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGAACTGCAGGTCATGGCAGCAAGGCTATGATGGCTGTAGGCCCTCAC |
| Internal bar code: | GGTAGATGTGGGCGAAAACATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3577 |
| LEAP-Seq percent confirming: | 98.9247 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 93 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATGCCATCCTCGTGGTTT |
| Suggested primer 2: | CAAGGCACAAGACACAAGGC |