Insertion junction: CLIP2.008245_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.008245
Chromosome:chromosome 2
Location:4167610
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g103900 MAPKKK11 (1 of 112) 2.7.11.25 - Mitogen-activated protein kinase kinase kinase / MLTK; Mitogen-Activated Protein Kinase Kinase Kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCCACACATGCAAGGCTGTACAGACCTTGCTCGTTTTCAGTATGTCGA
Internal bar code:AGTAATAGATTTTTCGGTGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:716
LEAP-Seq percent confirming:38.4615
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:AACTCGTCACGCTTGTTTGC
Suggested primer 2:CACATCGTGTGCATACGCTG