Insertion junction: CLIP2.008246_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.008246
Chromosome:chromosome 7
Location:3939730
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g339554 (1 of 1) K03372 - MFS transporter, PAT family, solute carrier family 33 (acetyl-CoA transportor), member 1 (ACATN, SLC33A1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCATGGGCCGCCGCAAGTCCTGGATCGTGCCGATCCAGCTCCTGACAGCT
Internal bar code:GATTCTAGGAAACGTTTATCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1760
LEAP-Seq percent confirming:95.8333
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:CAGACCCTCACCCCCATCTA
Suggested primer 2:GATCCCTGGATGACCTGTGC