Insertion junction: CLIP2.008294_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.008294
Chromosome:chromosome 17
Location:1530180
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g707050 UAF5,SPL12 U2 snRNP auxiliary factor associated protein; (1 of 1) K13093 - HIV Tat-specific factor 1 (HTATSF1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAGTTTCTCCTGTGCGGTGCGTACTGGGCCCCTGCCCCCGTGCCGTCCC
Internal bar code:CCGTTGAATGACCTCTAACATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1981
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:ATTACCTGTCGCAACCTGGG
Suggested primer 2:GCAAATCGACATCACAGGGC