| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.008302 |
| Chromosome: | chromosome 11 |
| Location: | 823263 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467639 | (1 of 1) PF04910 - Transcriptional repressor TCF25 (Tcf25) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTGGGTGGAGGCCATGTGGACTGTGCGGGCGCACGGGCGCTGGGTAC |
| Internal bar code: | TTAATAAAACAGTGTCGTTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2834 |
| LEAP-Seq percent confirming: | 95.8824 |
| LEAP-Seq n confirming: | 163 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 170 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATTTAGCCCACAGCCCAC |
| Suggested primer 2: | CCTCTGTGCTGTGTGTGGAT |