Insertion junction: CLIP2.008354_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.008354
Chromosome:chromosome 9
Location:5209237
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g406851 CDH1 Activator and specificity factor for anaphase promoting complex; (1 of 1) K03364 - cell division cycle 20-like protein 1, cofactor of APC complex (CDH1) gene_edge/mRNA_edge/3'UTR
Cre09.g406900 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGAAGGACTGATAACATTACAGGTGTGGGTGCACAGCACACTAGTGCTC
Internal bar code:CCTTCACCTCCGTAGGAGTCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:2079
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:50
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:50

Suggested primers for confirmation by PCR

Suggested primer 1:AGGGACAAGAGTTGAGTGCG
Suggested primer 2:AACACCCAAGGCAACCTCAA