Insertion junction: CLIP2.008354_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.008354
Chromosome:chromosome 9
Location:5209246
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g406851 CDH1 Activator and specificity factor for anaphase promoting complex; (1 of 1) K03364 - cell division cycle 20-like protein 1, cofactor of APC complex (CDH1) gene_edge/mRNA_edge/3'UTR
Cre09.g406900 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCGGGTCAGTTGCCCGCCCCCCCCCCCACACACACACACGAGGCACACG
Internal bar code:CCTTCACCTCCGTAGGAGTCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:675
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:AACACCCAAGGCAACCTCAA
Suggested primer 2:AGGGACAAGAGTTGAGTGCG