Insertion junction: CLIP2.008355_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.008355
Chromosome:chromosome 15
Location:5366286
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g734250 (1 of 57) 2.7.10.2 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCTGAGCCAAAGTCAACGTGCGCTGTGTTGTACTGCATTGCCGGAATC
Internal bar code:TTTCATTGACTCTCTTGAGAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:376
LEAP-Seq percent confirming:94.1176
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:CCACCCCATCCAACCATCAA
Suggested primer 2:CTGGCTAGCGCAAACCTACT