Insertion junction: CLIP2.008360_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.008360
Chromosome:chromosome 7
Location:2707781
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g331100 PHC11 Putative pherophorin-chlamydomonas homolog CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCATGGGGTTCATCATCGGGTTCATCATTGCGTTCATCATCGGGTTCAT
Internal bar code:TAAGAGTTTCGTTCGCCGAATC

Confirmation - LEAP-Seq

LEAP-Seq distance:2066
LEAP-Seq percent confirming:98.1132
LEAP-Seq n confirming:52
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:GCCCCCTGAACCTACTCAAC
Suggested primer 2:CTCCACCTCCACCTCCAGG