Insertion junction: CLIP2.008402_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.008402
Chromosome:chromosome 16
Location:7396134
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g680454 (1 of 8) PF13417 - Glutathione S-transferase, N-terminal domain (GST_N_3) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTACGCCTGCACGTGCGCATGACGCGTGGAGATTCAACAGCTGGGACTC
Internal bar code:CGTCAGGGATTGTACGCCTTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1752
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TTGAGACGTCGTGTTACCCG
Suggested primer 2:AACACATTGTTCGCGTGTGG