Insertion junction: CLIP2.008421_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.008421
Chromosome:chromosome 6
Location:1643968
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g262000 SNR5 SNARE-associated Golgi protein; (1 of 6) PF09335 - SNARE associated Golgi protein (SNARE_assoc) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAGCAGCACCACCGCAAAGGTGTGGAACAATAGGAATACATGTAGCGAG
Internal bar code:TGAAGCATAGTTATTCGGAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1975
LEAP-Seq percent confirming:92.4051
LEAP-Seq n confirming:73
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:79

Suggested primers for confirmation by PCR

Suggested primer 1:GCACGTACAGGGGAGCTATC
Suggested primer 2:GTCAGGTGTCAGGTGTGAGG