| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.008436 |
| Chromosome: | chromosome 9 |
| Location: | 5812547 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g411400 | MOT45 | PF13 protein required for dynein inner/outer arm assembly in the | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGAGTCGGCCGGGTGCAGCACACGTGCGGCCACGTGGTAGCCACCGGG |
| Internal bar code: | AAAGTGGTAGATCAGGGTGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1105 |
| LEAP-Seq percent confirming: | 70.0 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAATTGTACCGTGCACGCAC |
| Suggested primer 2: | CACCTCTCACACACCCTCAC |