Insertion junction: CLIP2.008453_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.008453
Chromosome:chromosome 2
Location:1584854
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g084900 RPAP3 RNA polymerase II-associated protein 3; (1 of 1) PF13414//PF13877 - TPR repeat (TPR_11) // Potential Monad-binding region of RPAP3 (RPAP3_C) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGAGGGCGCGGGGGCCACGGCGCCACCCGCGCGGCCGCGCGGCGCCGGG
Internal bar code:CGTGGTCTTGCAGATTTGGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1628
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:68
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:68

Suggested primers for confirmation by PCR

Suggested primer 1:CATGACAGGGCCTTGGACAT
Suggested primer 2:CACCTTCCACCGCTTCATCT