Insertion junction: CLIP2.008491_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.008491
Chromosome:chromosome 2
Location:5956318
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g118050 CYCU1 U-type cyclin; (1 of 1) PTHR15615:SF20 - CYCLIN FAMILY PROTEIN-RELATED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCTTCGGCTGGGGCGGCCCCTCATCTAACTGGAGAGTCAGCTGTACATG
Internal bar code:CTCACAAGAATGCCACAGAATA

Confirmation - LEAP-Seq

LEAP-Seq distance:1291
LEAP-Seq percent confirming:94.2308
LEAP-Seq n confirming:98
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:104

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCACTTGGTGAACTTGGC
Suggested primer 2:CACTCGCGAACTCGTATCGA