Insertion junction: CLIP2.008491_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.008491
Chromosome:chromosome 2
Location:5958264
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g118050 CYCU1 U-type cyclin; (1 of 1) PTHR15615:SF20 - CYCLIN FAMILY PROTEIN-RELATED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAAGTTTGACTAGTTTCTGTGAGGCCGGGCATCCTTTATTGCTTGGGTGC
Internal bar code:GCTTGCTCGCAGACGCTACTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:3045
LEAP-Seq percent confirming:97.7011
LEAP-Seq n confirming:255
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:261

Suggested primers for confirmation by PCR

Suggested primer 1:CACTCGCGAACTCGTATCGA
Suggested primer 2:CTGCACTTGGTGAACTTGGC