Insertion junction: CLIP2.008505_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.008505
Chromosome:chromosome 3
Location:2456410
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g159016 (1 of 13) 2.7.11.1//2.7.11.25 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase // Mitogen-activated protein kinase kinase kinase / MLTK intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCCCCTCTCAAAGGGCGCACCACCCACCATCCGTCCAACCAACGACCCA
Internal bar code:CTGATCTATTAAAGGTTGTGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:6134
LEAP-Seq percent confirming:88.5135
LEAP-Seq n confirming:131
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:148

Suggested primers for confirmation by PCR

Suggested primer 1:CGGGCGCTTTGATCAGATTG
Suggested primer 2:CAGAGAGAAACACCCACCCC