Insertion junction: CLIP2.008526_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.008526
Chromosome:chromosome 17
Location:1344066
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g705778 Chlamydomonas-specific family W protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAAAGAAGGCTGCTGCTCCCAGCCACAGACCCCACGCCTGCTTGTGGGG
Internal bar code:GTGTCATTCTTACGAATTGCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1420
LEAP-Seq percent confirming:3.0303
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:32
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:TCTCACCGGGTTTCCTTTCG
Suggested primer 2:GTGTCGACCCCTTCCATGTT