| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.008527 |
| Chromosome: | chromosome 5 |
| Location: | 2100431 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g236850 | NAT14 | N-acetyltransferase; (1 of 71) IPR016181 - Acyl-CoA N-acyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTCAGCTGCCCCGGACTCTGGATCCTCCGCGGCCTCAGATTCAGACTC |
| Internal bar code: | GGGGTAACATGGCCTAAAGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1771 |
| LEAP-Seq percent confirming: | 21.9512 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAGCGACAGTGACAGTGA |
| Suggested primer 2: | GGAATCCTTTTTGCGCCAGG |