Insertion cassette: | CIB2 |
Side of cassette: | 5' truncated? |
Strand: | + |
Strain: | CLIP2.008530 |
Chromosome: | chromosome 17 |
Location: | 3299830 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g722250 | RRM14 | Putative rRNA (guanine-N2-)-methyltransferase; (1 of 1) 2.1.1.173//2.1.1.264 - 23S rRNA (guanine(2445)-N(2))-methyltransferase // 23S rRNA (guanine(2069)-N(7))-methyltransferase / 23S rRNA m(7)G(2069) methyltransferase | CDS/intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGTGCAGTGGTGGCAGTATCGCGATGTGAGATGTTTCGGTAATTCAGT |
Internal bar code: | AAGTGATGGGGGTAGACAGTTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1480 |
LEAP-Seq percent confirming: | 57.1429 |
LEAP-Seq n confirming: | 4 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTGATCCCGCCTAGTGTAC |
Suggested primer 2: | ATGTTGTACACACCCGCCTT |