Insertion junction: CLIP2.008549_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.008549
Chromosome:chromosome 12
Location:8687776
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g547800 SNE15 (1 of 8) PTHR10366//PTHR10366:SF411 - NAD DEPENDENT EPIMERASE/DEHYDRATASE // HIGH CHLOROPHYLL FLUORESCENCE PHENOTYPE 173 PROTEIN; NAD dependent epimerase/dehydratase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCACCTCCCCAGGCTCTGTGGAGCGCTGCACTACCGTGCGGCGAGTGGT
Internal bar code:TTTAATATGCGGTAAAGTCAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:391
LEAP-Seq percent confirming:21.6667
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:47
LEAP-Seq n unique pos:60

Suggested primers for confirmation by PCR

Suggested primer 1:TTGCCGCTGACCAACATTTG
Suggested primer 2:CCATGGTCAAGGGAGTGGAC