| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.008556 |
| Chromosome: | chromosome 4 |
| Location: | 2056294 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g219350 | PLC1 | (1 of 14) IPR017946 - PLC-like phosphodiesterase, TIM beta/alpha-barrel domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTCGCATCAATACCGTACAGTGGCCTCATCACAGCCGCAGCCGCAGCC |
| Internal bar code: | GCATCGATGTAGGGTACAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 138 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGGGGGATGATCGACCTC |
| Suggested primer 2: | CCACACAGCCCCACATACAT |