| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.008578 |
| Chromosome: | chromosome 6 |
| Location: | 7041013 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g297400 | (1 of 1) 1.3.1.74 - 2-alkenal reductase (NAD(P)(+)) / NADPH:2-alkenal alpha,beta-hydrogenase | 5'UTR | |
| Cre06.g297450 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAAAGTTGTGCAGGCCCACAGAGCGTGACAATCGCGTGCCAACCGAGCT |
| Internal bar code: | ACAGCGTTCTAGGGGAACCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1430 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCGGGATAGGAGCAGAAG |
| Suggested primer 2: | ATCTTCACCATGCCCTTGGG |