| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.008587 |
| Chromosome: | chromosome 2 |
| Location: | 5166576 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g111950 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGCTCCTCCTATCCTCCTCCTAGGGCCCCCCTATCCTCCTCCTAGGG |
| Internal bar code: | TATGTCCTCGTGTGGGGTCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4206 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCGGCAGTAGCAGTACTAG |
| Suggested primer 2: | CAAAACCAGAACAGGTGCCG |