Insertion junction: CLIP2.008605_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.008605
Chromosome:chromosome 4
Location:3864232
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g230340 FAP206 (1 of 1) PTHR21442:SF0 - UPF0704 PROTEIN C6ORF165; Flagellar Associated Protein 206 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTGGTTTAGTTTGGGCTGCTGTCTACGACCCCCCCCGTCTCTCCTCAC
Internal bar code:TGGAGAGGCTCTGAGGTATATT

Confirmation - LEAP-Seq

LEAP-Seq distance:496
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGTGTGTGTGGGTGTGTA
Suggested primer 2:TTGCAACTAACGCCTCGACT