Insertion junction: CLIP2.008613_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.008613
Chromosome:chromosome 6
Location:4856908
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g280350 (1 of 5) IPR001680//IPR011047//IPR017986//IPR020472 - WD40 repeat // Quinonprotein alcohol dehydrogenase-like superfamily // WD40-repeat-containing domain // G-protein beta WD-40 repeat CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGAGTCCAGCGCCAGACAGCCCACCGCGTCCGTGTGCCCCGTGTGGCAG
Internal bar code:CGTCAATACGTCTAGGGAACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:460
LEAP-Seq percent confirming:11.1111
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:32
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:ATCCTGGCGCTCAAGTTTGA
Suggested primer 2:AAGCCAAGGAAGACAGACCG