| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.008619 |
| Chromosome: | chromosome 13 |
| Location: | 43439 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g801504 | (1 of 1) K03470 - ribonuclease HII (rnhB) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGAAGTCGTCCTCCTCCGCCGCCAGCAGGGGGGGAAGCGGCGGGCGAG |
| Internal bar code: | TGTCATGGTGAGCATTCCATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4889 |
| LEAP-Seq percent confirming: | 84.2105 |
| LEAP-Seq n confirming: | 112 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 133 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACAACAACAGGCTTGCGC |
| Suggested primer 2: | CACGGGCTGATTTTGCGAAA |