| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.008627 |
| Chromosome: | chromosome 8 |
| Location: | 3017843 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g375050 | FAP86 | Flagellar Associated Protein 86 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGAAACAGCACTCTTACGCTGTATTACTCTCAGCGGGCTCAGAGTGCA |
| Internal bar code: | TCCCTTAAAGGGTAGGGCACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 654 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAACGTGTGTGTGTGTGTC |
| Suggested primer 2: | TTCTGCCTGGCGCTCTTTTA |