| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.008627 |
| Chromosome: | chromosome 8 |
| Location: | 3017844 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g375050 | FAP86 | Flagellar Associated Protein 86 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGTTCCTGCGGGCGTCGGGAACAAAAGGGCATAGGTGAGGCCGCGGTA |
| Internal bar code: | TCCCTTAAAGGGTAGGGCACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2487 |
| LEAP-Seq percent confirming: | 90.6667 |
| LEAP-Seq n confirming: | 68 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTGCCTGGCGCTCTTTTA |
| Suggested primer 2: | CGAACGTGTGTGTGTGTGTC |