Insertion junction: CLIP2.008638_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.008638
Chromosome:chromosome 16
Location:3913982
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g689250 GTR23 (1 of 1) PF04564//PF06701 - U-box domain (U-box) // Mib_herc2 (MIB_HERC2); Putative ubiquitin-protein ligase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGACCGTGGCCAGCGCGCTGCTTTTCACACCCAGCGACCTGCTGCACC
Internal bar code:TTGTGTGTAAGTACATGACATA

Confirmation - LEAP-Seq

LEAP-Seq distance:963
LEAP-Seq percent confirming:96.1538
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:TCTTCAACAGCCACCGACTC
Suggested primer 2:CTGTTCGCGAGCACCAAAAA