| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.008655 |
| Chromosome: | chromosome 12 |
| Location: | 3712764 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g514000 | LAG1 | Predicted protein related to longevity assurance protein; (1 of 5) 2.3.1.24 - Sphingosine N-acyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCTTGACCGCAATCTCCACAATCTTGTAGAACCATATCAGCTGCAGAA |
| Internal bar code: | TACCGCGTATCATACTGGTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1916 |
| LEAP-Seq percent confirming: | 52.4691 |
| LEAP-Seq n confirming: | 85 |
| LEAP-Seq n nonconfirming: | 77 |
| LEAP-Seq n unique pos: | 162 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACATCATCCGCATGCAGG |
| Suggested primer 2: | CTCTACATCCCGTGTCGTCG |