| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.008660 |
| Chromosome: | chromosome 11 |
| Location: | 2328423 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095134 | (1 of 2) 2.1.1.9 - Thiol S-methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGGCCAGGTTTGACGCACCCATGACGGACAAAGGCGCTGCATGCTGCC |
| Internal bar code: | TATGGACAGTGTCTCGTGGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1973 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTTCCTCGGGAATCAGCC |
| Suggested primer 2: | GCATGATGCTCGGACGGATA |