Insertion junction: CLIP2.008668_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.008668
Chromosome:chromosome 2
Location:7110845
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g390134 (1 of 13) 2.7.11.1//2.7.11.25 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase // Mitogen-activated protein kinase kinase kinase / MLTK intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCTAGCTAGGCCGTGACGGCCGACCACCCGCAACGTCATGTCTACTGT
Internal bar code:CGCGAGGGTGTTCGTTAGTCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:4116
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:97
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:97

Suggested primers for confirmation by PCR

Suggested primer 1:ATGACGAGTGTGGTGGGAAC
Suggested primer 2:TTGTCGTCGTTGTCTGCGTA