Insertion junction: CLIP2.008670_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.008670
Chromosome:chromosome 7
Location:3657778
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g337450 GT90F9,GT90-9,CGL75 Glycosyl transferase GT90 family protein 9; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCACCCTCACCTCCCACGCCCCCTTCTCACCCCAACCTCCCATCCCCA
Internal bar code:ACAGTGATCTTTGTTGTTTGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1536
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TGACGTTGAGTTCGAAGGGG
Suggested primer 2:GCTACCCGAGTACGACATGG