| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.008712 |
| Chromosome: | chromosome 16 |
| Location: | 4315063 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g669050 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCATTCCACGTCCTCCCGAAACCGCCGCCGCCGCCGCTGATCGCCCC |
| Internal bar code: | ATTATCAATGAGTAGACTGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3199 |
| LEAP-Seq percent confirming: | 84.6154 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGATTCCGGCAGCAATGA |
| Suggested primer 2: | CGCCTTCTTGACCAGGTGAT |