| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.008735 |
| Chromosome: | chromosome 10 |
| Location: | 3180470 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g441850 | (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCGGCCCGCTGTACGGACTCCTGCTCCCGGCACACGCCAATGCGCGC |
| Internal bar code: | TTCTCCCTTACCCGCATGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 361 |
| LEAP-Seq percent confirming: | 35.2941 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCCTTACACGTGCTTTGC |
| Suggested primer 2: | CACAAAAACAGCATGGCGGA |