| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.008752 |
| Chromosome: | chromosome 1 |
| Location: | 5477305 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g038750 | MOT7 | (1 of 1) IPR007024 - BLUF domain; motile cilia related | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGAGGCGACTGGGTGTTTGGCAAGGGCCGTGTCACCGTGCCGTCCACAA |
| Internal bar code: | AAAGTCTAGCGAAAAACGTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2450 |
| LEAP-Seq percent confirming: | 7.14286 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAACCCTGTCCACTCGCAC |
| Suggested primer 2: | CTTGCTTGTGCCGAGCTTTT |