| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.008752 |
| Chromosome: | chromosome 1 |
| Location: | 5477515 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g038750 | MOT7 | (1 of 1) IPR007024 - BLUF domain; motile cilia related | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATATGGCAGATTAGCGTAGCACGCACAAGTTGTTCGGAGCGTGCGGTG |
| Internal bar code: | TGCCGTTCGCACGGCTTATGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4438 |
| LEAP-Seq percent confirming: | 3.0303 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGCTTGTGCCGAGCTTTT |
| Suggested primer 2: | TTAACCCTGTCCACTCGCAC |