| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.008754 |
| Chromosome: | chromosome 2 |
| Location: | 1278781 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g082200 | HSK1 | (1 of 3) 2.7.1.39 - Homoserine kinase; Homoserine kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTTGACGTGGTGGCCGCCTTGCTGGACATGCTGGTCACGGCCGCGGTG |
| Internal bar code: | GTTTGGTTGATCGGTGGGGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2195 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 52 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAGCACACCACTGTCAGG |
| Suggested primer 2: | GGGGTTCACGGTATTTGGGT |