| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.008754 |
| Chromosome: | chromosome 2 |
| Location: | 1278783 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g082200 | HSK1 | (1 of 3) 2.7.1.39 - Homoserine kinase; Homoserine kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTCCGCAGCCAATGCCCGCCTCTCCGCATCCAACCTAAGCCTCTGCTC |
| Internal bar code: | GTTTGGTTGATCGGTGGGGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1592 |
| LEAP-Seq percent confirming: | 97.3684 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGTTCACGGTATTTGGGT |
| Suggested primer 2: | ATGAGCACACCACTGTCAGG |