| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.008759 |
| Chromosome: | chromosome 16 |
| Location: | 4336857 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g668800 | MTA4 | Predicted protein; (1 of 2) PTHR24032:SF25 - SPORE COAT PROTEIN SP85 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTGACGCGTGGCCGCTGTCCCTGCCCCGGCATACACACACGCGACAC |
| Internal bar code: | CGGTGCATTAACTTTGGGAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2271 |
| LEAP-Seq percent confirming: | 84.8101 |
| LEAP-Seq n confirming: | 67 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 79 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGACTGTGACCTCCACCTG |
| Suggested primer 2: | ACAGCGGCCACGATACATAG |