| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.008769 |
| Chromosome: | chromosome 7 |
| Location: | 1266538 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g321951 | NRAMP2 | Manganese/metal transporter, NRAMP homolog; (1 of 2) PTHR11706//PTHR11706:SF33 - MANGANESE TRANSPORTER // NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCTCCCAGAAATGTCAGCGGACGGCTTGAGCGCCGAGGTTGGCACTT |
| Internal bar code: | TTAATTAGTGCCTTTTCTACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2087 |
| LEAP-Seq percent confirming: | 98.913 |
| LEAP-Seq n confirming: | 91 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 92 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGCTCTCCCGCCTATGAG |
| Suggested primer 2: | TTTCTGTGCTGGCTTTTCGC |