| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.008773 |
| Chromosome: | chromosome 10 |
| Location: | 1229217 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g426335 | (1 of 5) PF07496 - CW-type Zinc Finger (zf-CW) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAATTGCACGTCCGAGCGGCGACGACTGGGACGGCCAAGCGTGCAACCC |
| Internal bar code: | TCGAACACAGAGCTGTCTAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3158 |
| LEAP-Seq percent confirming: | 94.2029 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAATCATCTTGCGCTGCTCC |
| Suggested primer 2: | GTGTCGCCTCTCGTAGCTAC |