| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.008790 |
| Chromosome: | chromosome 5 |
| Location: | 3026859 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g231000 | CSA5 | Chlorophyceae-specific gene family A protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATATCACTGAGATGGGACAGACCTGGGGGACGTTGAAAGAACCATGTC |
| Internal bar code: | ACGTCGGCTAATGGGGCCAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1525 |
| LEAP-Seq percent confirming: | 94.2857 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGGTAAGCTGTCCGTCC |
| Suggested primer 2: | CAGTGTGATGCGTTTTGCCA |