Insertion junction: CLIP2.008797_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.008797
Chromosome:chromosome 17
Location:5191253
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g736850 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTCGCCTGGTTTCAGGGGGCCATTCTGGCGGATTTCGGGAAGCGCTCC
Internal bar code:GCACGTATTCGCGAGGGAGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1323
LEAP-Seq percent confirming:53.3333
LEAP-Seq n confirming:40
LEAP-Seq n nonconfirming:35
LEAP-Seq n unique pos:75

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTTTGCGGTGGTTGTGAG
Suggested primer 2:CTCTACTCGCAGTAGCTGGC