| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.008817 |
| Chromosome: | chromosome 12 |
| Location: | 4530712 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g521200 | RFC1 | (1 of 1) K10754 - replication factor C subunit 1 (RFC1); DNA replication factor C complex subunit 1 | 3'UTR/CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGACCTCCGCCGCGCCCACCCGCCGGCTTTGCCGCGGCGCCCGCTTTC |
| Internal bar code: | AATGTCCTTGTCTAGAACTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 954 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTCGCAAGCCTTATGTCG |
| Suggested primer 2: | TATCAGGCGCGACTGCTTAG |