| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.008828 |
| Chromosome: | chromosome 13 |
| Location: | 2244670 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g578451 | TSL1 | (1 of 2) 6.1.1.4 - Leucine--tRNA ligase / Leucyl-tRNA synthetase; Leucyl-tRNA synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTGGAACTGAAGTACACTTACGCCTGACCCCCCCTCCCCTGCCTACCC |
| Internal bar code: | TTAACTGCTTTCTTAGGGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 59 |
| LEAP-Seq percent confirming: | 3.22581 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 60 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCAACATGCCTGGCTGAG |
| Suggested primer 2: | CACCCAGGTCGTACAGAACC |