| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.008837 |
| Chromosome: | chromosome 3 |
| Location: | 418278 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144847 | PYK4 | (1 of 2) PTHR11817//PTHR11817:SF3 - PYRUVATE KINASE // PYRUVATE KINASE; Pyruvate kinase 4 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGTCATCACCGCCACCCAGATGCTGGAGTCCATGACCAGCGCGCCGCT |
| Internal bar code: | TTGACGGTAGCATTAATGTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2366 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAACGACATGGGAGGAGC |
| Suggested primer 2: | CAACCCGAAACATGCCTGTG |