| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.008839 |
| Chromosome: | chromosome 13 |
| Location: | 458772 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g564950 | EFG13 | (1 of 1) K14536 - ribosome assembly protein 1 (RIA1); Ribosomal protein S5/Elongation Factor G/III/V family protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGATACCTTCTCTATCGCACTCCATTCCGCGACCAGCCCCGATGACCC |
| Internal bar code: | ACGTTATGCTAGGAAATCGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 553 |
| LEAP-Seq percent confirming: | 11.7647 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAGAGGGCACAATCGGAC |
| Suggested primer 2: | CAAACAACGCGCACAAACAC |