Insertion junction: CLIP2.008843_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.008843
Chromosome:chromosome 8
Location:4375800
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g384950 (1 of 1) K14398 - cleavage and polyadenylation specificity factor subunit 6/7 (CPSF6_7) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTAGTGCCTCAGACCGAGACCCGCCGAATCCCCGCGAACACTCACTTGC
Internal bar code:AGTTTTAATGGTCTGGAGATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:2767
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:56
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:56

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTCAACACACAGCACTGC
Suggested primer 2:TCCTTTGGTTCCTCCATGCC