| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.008844 |
| Chromosome: | chromosome 2 |
| Location: | 1266862 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g082050 | (1 of 1) K13115 - coiled-coil domain-containing protein 130 (CCDC130) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAACCTGCACCCGCGATTGCGTGCGTAGGGTGCTCGTGTTGATAGGAAG |
| Internal bar code: | GATTGAAAATTTGCAGGTTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 205 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGGTGCCCTTCAACATCT |
| Suggested primer 2: | CTCTTGCGGGCAGTGTTTTG |